E-GEOD-16442 - microRNA (miRNA) expression from human 293 cells

Status
Submitted on 3 June 2009, released on 22 May 2010, last updated on 1 May 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (8)
Description
Expression phospho-mimic TRBP enhanced in vitro miRNA production and cellular expression of ectopic miRNA. We used miRNA microarray experiments to determine the effect of expression of phopsho-mimic TRBP on global endogenous miRNA expression. We established isogenic cell lines stably expressing phospho-mutant and phospho-mimic TRBP using Flp-In 293 cells and a Flp Recombinase Target system. After monoclonal cell colonies were selected, cells were cultured for two weeks. Total RNA was harvested when cells were ~75% confluent. Initial analysis was performed using one sample from each experimental group. Subsequent analysis included an additional two samples for each group. miRNA with values less than 500 were not included in analysis.
Experiment type
transcription profiling by array 
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-16442.idf.txt
Sample and data relationshipE-GEOD-16442.sdrf.txt
Raw data (1)E-GEOD-16442.raw.1.zip
Processed data (1)E-GEOD-16442.processed.1.zip
Array designA-GEOD-8658.adf.txt
Links