Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-16356 - RNAi knock down of MAFin human lymphatic endothelial cells

Status
Submitted on 31 May 2009, released on 10 October 2009, last updated on 10 June 2011
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (5)
Description
Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple MAF-downregulating microRNA. Here we test the effects of MAF silencing by siRNA in LEC cells using Affymetrix hgu133plus2 chips. Experiment Overall Design: There are n=3 of 1. LEC control cells transfected with a non-targeting siRNA, 2. LEC transfected with a MAF-targeting siRNA
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-16356.idf.txt
Sample and data relationshipE-GEOD-16356.sdrf.txt
Raw data (1)E-GEOD-16356.raw.1.zip
Processed data (1)E-GEOD-16356.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-16356.eSet.r
Links