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E-GEOD-16250 - PBMCs exposed to the Mycobacterium tuberculosis H37Ra strain

Status
Released on 28 May 2014, last updated on 2 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (5)
Description
Human peripheral blood mononuclear cells were cultured in presence of H37Ra strain at 37oC, 5%CO2. Cellular aggregates were collected at 24h, and RNA extracted and hybridized to Affymetrix microarrays (HG-U133). Raw data from microarray experiments was analyzed with dCHIP and SAM programs to determine the significance of changes at the biological context. PBMCs were exposed in triplicate to H37Ra strain. PBMCs from the same donor were cultured in triplicate at the same conditions than treated samples. RNA was extracted from each sample and hybridized to Affymetrix Human arrays.
Experiment type
transcription profiling by array 
Contacts
Niradiz Reyes <geo@ncbi.nlm.nih.gov>, Alfonso Bettin, Ismael Reyes, Jan Geliebter
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-16250.idf.txt
Sample and data relationshipE-GEOD-16250.sdrf.txt
Raw data (1)E-GEOD-16250.raw.1.zip
Processed data (1)E-GEOD-16250.processed.1.zip
Array designA-AFFY-44.adf.txt
Links