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E-GEOD-16014 - Transcription profiling of human monocytic leukemia cell line THP-1 treated with Ganoderma lucidum polysaccharides F3

Status
Submitted on 7 May 2009, released on 16 May 2009, last updated on 10 June 2011
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (5)
Description
In order to identify patterns of gene expression associated with biological effects in THP-1 cells induced by F3, we performed a transcriptomic analysis on the THP-1 control and F3-treated THP-1 cells by oligonucleotide microarray Experiment Overall Design: 10^7 cells/mL concentrations of THP-1 cells were seeded in 100 mm dish and incubated overnight. After that, cells were treated with F3 at a final concentration of 30 ug/mL. After incubated for 6 and 24 hours, the cell pellets were collected by centrifugation at 250g for 5 min, correspondingly. Controlled samples of un-induced cells were treated in the same way with the same amount of medium.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Hsueh-Fen Juan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-16014.idf.txt
Sample and data relationshipE-GEOD-16014.sdrf.txt
Raw data (1)E-GEOD-16014.raw.1.zip
Processed data (1)E-GEOD-16014.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-16014.eSet.r
Links