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E-GEOD-15653 - Microarray analysis of surgical liver biopsies from 13 obese (nine with type 2 diabetes) and five control subjects

Status
Released on 1 June 2009, last updated on 6 August 2018
Organism
Homo sapiens
Samples (18)
Array (1)
Protocols (6)
Description
Hepatic lipid accumulation is an important complication of obesity linked to risk for type 2 diabetes. To identify novel transcriptional changes in human liver which could contribute to hepatic lipid accumulation and associated insulin resistance and type 2 diabetes (DM2), we evaluated gene expression and gene set enrichment in surgical liver biopsies from 13 obese (9 with DM2) and 5 control subjects, obtained in the fasting state at the time of elective abdominal surgery for obesity or cholecystectomy. RNA was isolated for cRNA preparation and hybridized to Affymetrix U133A microarrays. Human liver samples were obtained from 5 lean control subjects undergoing elective cholecystectomy and 13 obese subjects (with or without Type 2 diabetes) undergoing gastric bypass surgery. Subjects with diabetes were classified as either well-controlled or poorly-controlled.
Experiment type
transcription profiling by array 
Contacts
Mary Elizabeth Patti <mary.elizabeth.patti@joslin.harvard.edu>, Allison B Goldfine, Edward Mun, Joshua Schroeder, Jussi Pihlajamäki, Mary-Elizabeth Patti
Citation
Thyroid hormone-related regulation of gene expression in human fatty liver. Pihlajamaki J, Boes T, Kim EY, Dearie F, Kim BW, Schroeder J, Mun E, Nasser I, Park PJ, Bianco AC, Goldfine AB, Patti ME. , PMID:19549744
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-15653.idf.txt
Sample and data relationshipE-GEOD-15653.sdrf.txt
Raw data (1)E-GEOD-15653.raw.1.zip
Processed data (1)E-GEOD-15653.processed.1.zip
Array designA-AFFY-33.adf.txt
Links