E-GEOD-15646 - RNAi profiling of human Kasumi-1 AML1-ETO knockdown samples

Status
Submitted on 13 April 2009, released on 11 May 2009, last updated on 10 June 2011
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (5)
Description
Kasumi-1 AML cells that were transfected in triplicate with AML1-ETO or luciferase siRNA constructs by either Amaxa nucleofection or Biorad siLentFect and incubated for 96 hours. Microarrays used to discover an AML1-ETO signature for a GE-HTS screen to identify AML1-ETO modulators. Experiment Overall Design: Kasumi-1 AML cells incubated for 96 hours after they were transfected in triplicate with AML1-ETO or luciferase siRNA constructs by either Amaxa nucleofection or Biorad siLentFect along with three control samples not transfected with a construct.
Experiment types
RNAi profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-15646.idf.txt
Sample and data relationshipE-GEOD-15646.sdrf.txt
Raw data (1)E-GEOD-15646.raw.1.zip
Processed data (1)E-GEOD-15646.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-15646.eSet.r
Links