E-GEOD-15646 - RNAi profiling of human Kasumi-1 AML1-ETO knockdown samples
Submitted on 13 April 2009, released on 11 May 2009, last updated on 10 June 2011
Kasumi-1 AML cells that were transfected in triplicate with AML1-ETO or luciferase siRNA constructs by either Amaxa nucleofection or Biorad siLentFect and incubated for 96 hours. Microarrays used to discover an AML1-ETO signature for a GE-HTS screen to identify AML1-ETO modulators. Experiment Overall Design: Kasumi-1 AML cells incubated for 96 hours after they were transfected in triplicate with AML1-ETO or luciferase siRNA constructs by either Amaxa nucleofection or Biorad siLentFect along with three control samples not transfected with a construct.
RNAi profiling by array, unknown experiment type