E-GEOD-15624 - Transcription profiling of mouse T cells treated with halofuginone to investigate regulation of Y cell gene expression by Halofuginone (HF)

Submitted on 10 April 2009, released on 19 April 2009, last updated on 10 June 2011
Mus musculus
Samples (12)
Array (1)
Protocols (5)
T cell differentiation to the Th17 effector subset requires stimulation through the T cell and co-stimulatory receptors, together with cytokine stimulation by TGFb and IL-6. The small molecule halofuginone (HF) inhibits Th17 cell development and induces a pattern of stress-regulated gene expression that mimics amino acid starvation. We used global transcript profiling to ask how halofuginone modulates gene expression induced during T cell activaiton and Th17 differentiation Experiment Overall Design: Purified mouse CD4+ CD25- T cells were activated under Th17 polarizing cytokine conditions and treated with either halofuginone or its inactive derivative, MAZ1310, for 3- or 6-hours.
Experiment types
transcription profiling by array, unknown experiment type
Investigation descriptionE-GEOD-15624.idf.txt
Sample and data relationshipE-GEOD-15624.sdrf.txt
Raw data (1)E-GEOD-15624.raw.1.zip
Processed data (1)E-GEOD-15624.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-15624.eSet.r