Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-1559 - Transcription profiling of mouse HSC at various time points after treatment with 5-Fluorouracil

Status
Released on 15 November 2007, last updated on 1 May 2014
Organism
Mus musculus
Samples (15)
Array (1)
Protocols (6)
Description
HSC (Sca+ SP) were isolated from 8-12 week C57B6 mice at various time points after treatment with 5-Fluorouracil. RNA was isolated from 50,000-100,000 FACS sorted cells and subjected to two rounds of T7 based linear amplification using Ambion's Message Amp kit. Two replicates from each time point were analyzed. http://www.plosbiology.org/plosonline/?request=get-document&doi=10.1371%2Fjournal.pbio.0020301
Experiment types
transcription profiling by array, co-expression, compound treatment, organism part comparison, time series
Contact
Citation
Molecular signatures of proliferation and quiescence in hematopoietic stem cells. Teresa A Venezia, Akil A Merchant, Carlos A Ramos, Nathan L Whitehouse, Andrew S Young, Chad A Shaw, Margaret A Goodell. PLoS Biol 2(10):e301 (2004)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-1559.idf.txt
Sample and data relationshipE-GEOD-1559.sdrf.txt
Raw data (1)E-GEOD-1559.raw.1.zip
Processed data (1)E-GEOD-1559.processed.1.zip
Array designA-AFFY-6.adf.txt
R ExpressionSetE-GEOD-1559.eSet.r
Links