E-GEOD-15283 - Profiling of FACs sorted subpopulations of iPS cells from cell surface marker profile
Released on 7 June 2013, last updated on 2 June 2014
Two independent induced pluripotent stem cell lines: ES4CL1 (derived from foreskin) and MR90C2 (derived from lung Fibroblast) were maintained on inactivated mouse embryonic fibroblast (MEF) feeder cells in the presence of ESC media containing DMEM, 20% knock-out serum replacement with 100ng/mL of bFGF. 7 days after seeding, cells were harvested in TrypLE Express and FACs sorted using antibodies detecting the presence of cell surface antigens GCTM-2 and CD9. 4 independent fractions were isolated after cell sorting: P4 (CD9-Negative, GCTM-2 Negative); P5 (CD9-Low, GCTM-2 Low); P6 (CD9-Medium, GCTM-2 medium) and P7 (CD9-High, GCTM-2 High). Each individual experiment was performed in triplicate. Keywords: cell type comparison Three consecutive passages of both ES4CL1 and MR90C2 cells were grown in standard conditions, FACs sorted cells collected, and total RNA isolated. Microarray was performed on a total of 24 samples, (3 replicates of 4 FACs sorted populations from two independent cell lines).
transcription profiling by array
Sean Grimmond <email@example.com>, Gabriel Kolle
Identification of Unsafe Human Induced Pluripotent Stem Cell Lines Using a Robust Surrogate Assay for Pluripotency. Carlos Polanco J, Ho MS, Wang B, Zhou Q, Wolvetang E, Mason E, Wells CA, Kolle G, Grimmond SM, Bertoncello I, O'Brien C, Laslett AL. , Europe PMC 23728894