6 protocols
AccessionType
bioassay_data_transformation
ID_REF = Entrez Gene
VALUE = RMA calculated intensity from MBNI custom annotation
image_aquisition
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
nucleic_acid_extraction
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
feature_extraction
The data were analyzed with custom CDF environments created by the MBNI - The version 6 entrez gene ID CDF was used.
labeling
10 ug of Qiagen-purified total RNA
hybridization
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.