ID_REF = Entrez Gene
VALUE = RMA calculated intensity from MBNI custom annotation
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
The data were analyzed with custom CDF environments created by the MBNI - The version 6 entrez gene ID CDF was used.
10 ug of Qiagen-purified total RNA
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.