E-GEOD-14507 - Regulation of epidermal growth factor receptor signaling in human cancer cells by microRNA-7
Released on 23 January 2009, last updated on 27 June 2012
The epidermal growth factor receptor (EGFR) is frequently overexpressed in cancer and is an important therapeutic target. Aberrant expression and function of microRNAs has been associated with tumorigenesis. Bioinformatic predictions suggest that the human EGFR mRNA 3’-untranslated region contains three microRNA-7 (miR-7) target sites, which are not conserved across mammals. We found that miR-7 down-regulates EGFR mRNA and protein expression in cancer cell lines (lung, breast, and glioblastoma) via two of the three sites, inducing cell cycle arrest and cell death. Because miR-7 was shown to decrease EGFR mRNA expression, we used microarray analysis to identify additional mRNA targets of miR-7. These included Raf1 and multiple other genes involved in EGFR signaling and tumorigenesis. Furthermore, miR-7 attenuated activation of protein kinase B (Akt) and extracellular signal-regulated kinase 1/2 (ERK1/2), two critical effectors of EGFR signaling, in different cancer cell lines. These data establish an important role for miR-7 in controlling mRNA expression and indicate that miR-7 has the ability to coordinately regulate EGFR signaling in multiple human cancer cell types. Four samples are analysed: two biological replicates of A549 cells treated with miR-7 precursor and two biological replicates of A549 cells treated with miR-NC negative control precursor.
transcription profiling by array
Rebecca Webster <email@example.com>, John S Mattick, Karina J Price, Keith M Giles, Peter J Leedman, Priscilla M Zhang, Rebecca J Webster
Regulation of epidermal growth factor receptor signaling in human cancer cells by microRNA-7. Webster RJ, Giles KM, Price KJ, Zhang PM, Mattick JS, Leedman PJ. , Europe PMC 19073608