E-GEOD-13638 - Ago2 or Dicer knockdown effects on mRNA levels in HeLa

Status
Submitted on 18 November 2008, released on 4 February 2009, last updated on 10 June 2011
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (8)
Description
Analysis of mRNA changes in HeLa cells following Ago2 or Dicer depletion. Dicer, a cytoplasmic RNase III, generates the mature form of small RNAs including microRNA. Ago2 is a component of an effector complex of microRNA. Keywords: gene expression array-based (RNA / in situ oligonucleotide) siRNA against Ago2 or Dicer were trasnfected into HeLa cells. siRNA against GFP was used as a control. Biologically duplicated total RNAs were prepared from HeLa cells, 24 hrs and 48 hrs after siRNA transfection.
Experiment type
transcription profiling by array 
Contacts
Jinju Han, V N Kim, Young-Kook Kim
Citation
Posttranscriptional crossregulation between Drosha and DGCR8. Han J, Pedersen JS, Kwon SC, Belair CD, Kim YK, Yeom KH, Yang WY, Haussler D, Blelloch R, Kim VN.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13638.idf.txt
Sample and data relationshipE-GEOD-13638.sdrf.txt
Raw data (1)E-GEOD-13638.raw.1.zip
Processed data (1)E-GEOD-13638.processed.1.zip
Array designA-AFFY-141.adf.txt
R ExpressionSetE-GEOD-13638.eSet.r
Links