E-GEOD-13638 - Ago2 or Dicer knockdown effects on mRNA levels in HeLa
Submitted on 18 November 2008, released on 4 February 2009, last updated on 10 June 2011
Analysis of mRNA changes in HeLa cells following Ago2 or Dicer depletion. Dicer, a cytoplasmic RNase III, generates the mature form of small RNAs including microRNA. Ago2 is a component of an effector complex of microRNA. Keywords: gene expression array-based (RNA / in situ oligonucleotide) siRNA against Ago2 or Dicer were trasnfected into HeLa cells. siRNA against GFP was used as a control. Biologically duplicated total RNAs were prepared from HeLa cells, 24 hrs and 48 hrs after siRNA transfection.
transcription profiling by array
Jinju Han, V N Kim, Young-Kook Kim
Posttranscriptional crossregulation between Drosha and DGCR8. Han J, Pedersen JS, Kwon SC, Belair CD, Kim YK, Yeom KH, Yang WY, Haussler D, Blelloch R, Kim VN.