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E-GEOD-1361 - Concurrent Versus Individual Binding of HuR and AUF1 to Common Labile Target mRNAs

Status
Submitted on 3 May 2004, released on 9 June 2010, last updated on 27 March 2012
Organism
Homo sapiens
Samples (9)
Array (1)
Protocols (1)
Description
RNA was isolated from material that had been immunoprecipitated (IP) from Hela cells using antibodies recognizing RNA-binding proteins HuR or AUF1, as well as using a control IgG1 antibody. RNA was reverse-transcribed in the presence of [alpha-33P]dCTP and the radiolabeled product used to hybridize human cDNA arrays. The experiment was repeated using three independent sample sets. The samples were numbered HuR-1, HuR-2, HuR-3, AUF1-1, AUF1-2, AUF1-3, IgG1-1, IgG1-2, IgG1-3. HuR represents RNA from IP reactions using an anti-HuR antibody, AUF1 represents RNA from IP reactions using an anti-AUF1 antibody and, IgG1 represents RNA from IP reactions using an anti-IgG1 antibody. The numbers 1, 2 and 3 correspond to the three independent experimental datasets. Keywords = RNa-binding protein Keywords = mRNA stability Keywords = exosome Keywords = polysome Keywords = RNA motif Keywords: ordered
Experiment type
transcription profiling by array 
Contacts
Myriam Gorospe <myriam-gorospe@nih.gov>, Tomoko Kawai <kawaito@basic.med.tokushima-u.ac.jp>, Ashish Lal, Jennifer L Martindale, Krystyna Mazan-Mamczarz, Xiaoling Yang
Citation
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs. Lal A, Mazan-Mamczarz K, Kawai T, Yang X, Martindale JL, Gorospe M.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-1361.idf.txt
Sample and data relationshipE-GEOD-1361.sdrf.txt
Processed data (1)E-GEOD-1361.processed.1.zip
Array designA-GEOD-1211.adf.txt
Links