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E-GEOD-13469 - Transcription profiling by array of Arabidopsis expressing PAP1 under the control of the 35S promoter

Status
Released on 15 November 2008, last updated on 8 February 2012
Organism
Arabidopsis thaliana
Samples (10)
Array (1)
Protocols (3)
Description
Examine global gene expression patterns in control and 35S:PAP1 Arabidopsis plants upon environmental perturbation (light and temperature) over the course of the experiments. Experiment Overall Design: Red coloured 35S:PAP1, and empty vector control, plants of Arabidopsis thaliana Columbia were grown under room temperature, high light (RTHL, 22°C, 440 µmol m-2 s-1 irradiance) conditions to promote red leaf colouration. The growth conditions were then changed to a high day temperature, low light (HTLL: 30°C day, 150 µmol m-2 s-1 irradiance) stress treatment for six days, during which time the 35S:PAP1 leaves lost much of their red colouration, turning green. The growth conditions were then returned to a low temperature high light (LTHL, 15°C, 62% RH, 440 µmol m-2 s-1 irradiance) regime to restore the red colouration.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Environmental regulation of leaf colour in red 35S:PAP1 Arabidopsis thaliana. Rowan DD, Cao M, Lin-Wang K, Cooney JM, Jensen DJ, Austin PT, Hunt MB, Norling C, Hellens RP, Schaffer RJ, Allan AC. , PMID:19192188
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13469.idf.txt
Sample and data relationshipE-GEOD-13469.sdrf.txt
Raw data (1)E-GEOD-13469.raw.1.zip
Processed data (1)E-GEOD-13469.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-13469.eSet.r
Links