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E-GEOD-13411 - Gene expression by human splenic B-cell subsets

Status
Released on 30 December 2008, last updated on 27 March 2012
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (8)
Description
Enhanced secondary Ab responses are a vital component of adaptive immunity, yet little is understood about the intrinsic and extrinsic regulators of naïve and memory B cells that results in differences in their responses to Ag. Microarray analysis, together with surface and intracellular phenotyping, revealed that memory B cells have increased expression of members of the TNF receptor, SLAM, B7 and Bcl2 families, as well as the TLR-related molecule CD180 (RP105). Accordingly, memory B cells exhibited enhanced survival, proliferation and Ig secretion, as well as entered division more rapidly than naïve B cells in response to both T-dependent and T-independent stimuli. Furthermore, both IgM and isotype switched memory B cells, but not naïve B cells, co-stimulated CD4+ T cells in vitro through a mechanism dependent on their constitutive expression of CD80 and CD86. This study demonstrates that upregulation of genes involved in activation, co-stimulation and survival provides memory B cells with a unique ability to produce enhanced immune responses and contributes to the maintenance of the memory B cell pool. Keywords: cell type comparison Four subsets of human splenic B cells (naïve, IgM-memory, Ig isotype switched memory and plasma cells); sort-purified and analysed immediately ex vivo; performed in duplicate.
Experiment type
transcription profiling by array 
Contacts
Stuart Tangye <s.tangye@garvan.org.au>, Kim L Good, Stuart G Tangye
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13411.idf.txt
Sample and data relationshipE-GEOD-13411.sdrf.txt
Raw data (1)E-GEOD-13411.raw.1.zip
Processed data (1)E-GEOD-13411.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-13411.eSet.r
Links