E-GEOD-13383 - Transcription profiling of Arabidopsis dark 7-day-old Arabidopsis whole seedlings vs. 1h red light

Status
Submitted on 28 October 2008, released on 18 April 2009, last updated on 10 June 2011
Organism
Arabidopsis thaliana
Samples (2)
Array (1)
Protocols (5)
Description
Red light can affect a variety of responses in Arabidopsis. We characterize the early gene expression patterns of seedlings exposed to 1 hour of red light using a small sized sample of 5, 7-day-old seedlings and also performed dark controls. Methods that were developed for tissue extraction and labeling for microarrays were tested using these samples Experiment Overall Design: Only 5, 7 day-old dark-grown Arabidopsis seedlings were treated as described and harvested for RNA extraction and hybridization on Affymetrix microarrays. We pooled samples from one Petri plate with MS medium 2% sucrose. Treatment was 1 hour of red light 630 nm (max) and dark controls
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13383.idf.txt
Sample and data relationshipE-GEOD-13383.sdrf.txt
Raw data (1)E-GEOD-13383.raw.1.zip
Processed data (1)E-GEOD-13383.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-13383.eSet.r
Links