E-GEOD-13335 - Transcription profiling of mouse cells sensitive to necrosis (L929) and cells sensitive to apoptosis (NIH3T3)

Status
Submitted on 23 October 2008, released on 13 November 2008, last updated on 4 May 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (4)
Description
To explore gene expression profiles of cells sensitive to necrosis (such as L929 cells) and those sensitive to apoptosis (such as NIH3T3 cells), we conducted expression microarray analysis of L929 cells and NIH3T3 cells. Experiment Overall Design: Total RNA of 2 samples of unstimulated L929 cells or 2 samples of unstimulated NIH3T3 cells were extracted by Trizol followed by RNeasy. The quantity, purity and integrity of RNA were evaluated by UV spectrophotometry and RNA-nano Bioanalyzer. Sample processing and hybridization on Mouse Genome 430 2.0 GeneChip microarrays (Affymetrix) were performed according to manufacturer’s instructions. Genes that are differentially expressed in L929 cells relative to NIH3T3 cells were identified as having an FDR-adjusted p-value <0.05 and a fold-change >1.5.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13335.idf.txt
Sample and data relationshipE-GEOD-13335.sdrf.txt
Raw data (1)E-GEOD-13335.raw.1.zip
Processed data (1)E-GEOD-13335.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-13335.eSet.r
Links