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E-GEOD-13206 - Human shSIRT6 TNF-alpha timecourse

Submitted on 14 October 2008, released on 24 June 2010, last updated on 4 May 2014
Homo sapiens
Samples (16)
Array (1)
Protocols (18)
We established HeLa cell lines stably expressing a short hairpin RNA against SIRT6 (S6 sh2), and then completed genome-wide microarray analysis in shSIRT6 knock-down and control pSR HeLa cells. Prior to RNA extraction, HeLa cells were treated with TNF-alpha (5 ng/ml) for the indicated times. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Ambion MessageAmp RNA Amplification kit. Amplified human Universal Reference RNA (Stratagene) was used as reference RNA for analysis. Construction and array hybridization of human cDNA microarrays were performed as previously described (Perou et al., 2000). Groups of assays that are related as part of a time series. Compound Based Treatment: 5 ng/ml TNF-alpha Keywords: time_series_design Computed
Experiment type
transcription profiling by array 
SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span. Kawahara TL, Michishita E, Adler AS, Damian M, Berber E, Lin M, McCord RA, Ongaigui KC, Boxer LD, Chang HY, Chua KF.
Investigation descriptionE-GEOD-13206.idf.txt
Sample and data relationshipE-GEOD-13206.sdrf.txt
Processed data (1)
Array designA-GEOD-7490.adf.txt