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E-GEOD-13196 - Transcription profiling of zebrafish pineal gland

Status
Released on 11 May 2009, last updated on 10 June 2011
Organism
Danio rerio
Samples (14)
Array (1)
Protocols (5)
Description
Microarray data allowed detection of genes that are highly expressed in the pineal gland. Experiment Overall Design: Adult Tg(aanat2:EGFP)Y8 transgenic zebrafish in which EGFP marks the pineal gland were used for RNA extraction and hybridization on Affymetrix microarrays. Fish were anesthetized in 1.5 mM Tricane (Sigma) and sacrificed by decapitation, and pineal glands were removed under a fluorescent dissecting microscope. Since the pineal gland is a clock-containing organ and levels of certain transcripts may vary throughout the circadian cycle, glands were collected throughout the 24-hr cycle at 4 hr intervals. During the 24-hr cycle the fish were either maintained in a 12-h light/12-h dark cycle (LD) or kept in constant darkness (DD). 12 pineal glands were collected and pooled at each time point at each light condition, and total RNA was extracted (RNeasy, Qiagen).
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13196.idf.txt
Sample and data relationshipE-GEOD-13196.sdrf.txt
Raw data (1)E-GEOD-13196.raw.1.zip
Processed data (1)E-GEOD-13196.processed.1.zip
Array designA-AFFY-38.adf.txt
R ExpressionSetE-GEOD-13196.eSet.r
Links