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E-GEOD-13154 - miRNA profiling in male and female chicken embryonic gonads during sexual differentiation

Status
Released on 18 May 2010, last updated on 27 March 2012
Organism
Gallus gallus
Samples (25)
Array (1)
Protocols (6)
Description
MicroRNAs (miRNAs) are a highly conserved class of small RNAs which function in a sequence-specific manner to post-transcriptionally regulate expression of target genes. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryonic development, but a role for miRNAs in gonadal development and sex differentiation has not yet been reported. Using the chicken embryo as a vertebrate model, differential miRNA expression between male and female embryonic gonads, was analysed at three developmental stages (embryonic days (E) 5.5, E6.5 and E9.5), using custom-designed 4x2K CombiMatrix miRNA microarray. The aims of this study were to: 1-identify miRNAs differentialy expressed by sex; 2-identify sex-specific miRNAs; 3-analyse global changes in miRNA up-regulation in male versus female gonads before, during and after the histological onset of sexual differentiation. This study provides a basis for establishing whetehr miRNAs are involved in either initiating or regulating vertebrate gonadal sex differentiation. Keywords: miRNA, sex comparison, developmental stage comparison. miRNA samples from male and female embryonic chicken gonads from three developmental stages: embryonic day (E) 5.5 (Hamilton & Hamburger (HH) stage 27-28), E6.5 (HH stage 29-30) & E9.5 (HH stage 35-36). Samples are listed with biological replicates used for analysis in brackets following: 1 - Male E5.5 (5); 2 - Female E5.5 (4); 3 - Male E6.5 (5); 4 - Female E6.5 (3); 5 - Male E9.5 (4); 6 - Female E9.5 (4).
Experiment type
transcription profiling by array 
Contacts
Stephanie C Bannister <stephanie.bannister@csiro.au>, Andrew H Sinclair, Craig A Smith, Mark L Tizard, Timothy J Doran
Citation
Sexually dimorphic microRNA expression during chicken embryonic gonadal development. Bannister SC, Tizard ML, Doran TJ, Sinclair AH, Smith CA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13154.idf.txt
Sample and data relationshipE-GEOD-13154.sdrf.txt
Raw data (1)E-GEOD-13154.raw.1.zip
Processed data (1)E-GEOD-13154.processed.1.zip
Array designA-GEOD-7472.adf.txt
Links