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E-GEOD-13154 - miRNA profiling in male and female chicken embryonic gonads during sexual differentiation

Status
Submitted on 9 October 2008, released on 18 May 2010, last updated on 27 March 2012
Organism
Gallus gallus
Samples (25)
Array (1)
Protocols (6)
Description
MicroRNAs (miRNAs) are a highly conserved class of small RNAs which function in a sequence-specific manner to post-transcriptionally regulate expression of target genes. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryonic development, but a role for miRNAs in gonadal development and sex differentiation has not yet been reported. Using the chicken embryo as a vertebrate model, differential miRNA expression between male and female embryonic gonads, was analysed at three developmental stages (embryonic days (E) 5.5, E6.5 and E9.5), using custom-designed 4x2K CombiMatrix miRNA microarray. The aims of this study were to: 1-identify miRNAs differentialy expressed by sex; 2-identify sex-specific miRNAs; 3-analyse global changes in miRNA up-regulation in male versus female gonads before, during and after the histological onset of sexual differentiation. This study provides a basis for establishing whetehr miRNAs are involved in either initiating or regulating vertebrate gonadal sex differentiation. Keywords: miRNA, sex comparison, developmental stage comparison. miRNA samples from male and female embryonic chicken gonads from three developmental stages: embryonic day (E) 5.5 (Hamilton & Hamburger (HH) stage 27-28), E6.5 (HH stage 29-30) & E9.5 (HH stage 35-36). Samples are listed with biological replicates used for analysis in brackets following: 1 - Male E5.5 (5); 2 - Female E5.5 (4); 3 - Male E6.5 (5); 4 - Female E6.5 (3); 5 - Male E9.5 (4); 6 - Female E9.5 (4).
Experiment type
transcription profiling by array 
Contacts
Stephanie C Bannister <stephanie.bannister@csiro.au>, Andrew H Sinclair, Craig A Smith, Mark L Tizard, Timothy J Doran
Citation
Sexually dimorphic microRNA expression during chicken embryonic gonadal development. Bannister SC, Tizard ML, Doran TJ, Sinclair AH, Smith CA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13154.idf.txt
Sample and data relationshipE-GEOD-13154.sdrf.txt
Raw data (1)E-GEOD-13154.raw.1.zip
Processed data (1)E-GEOD-13154.processed.1.zip
Array designA-GEOD-7472.adf.txt
Links