E-GEOD-13120 - Transcription profiling of mouse neocortex to investigate age-related gene expression changes
Submitted on 8 October 2008, released on 13 January 2009, last updated on 27 March 2012
Aging is associated with major nuclear changes affecting genomic integrity and gene expression. Here we compare the gene expression profiles in the neocortex of young (5 months old) and old (30 months old) B6xC3 F1 mice. In a related study, we compare genes that change expression with age to SIRT1 associated genes identified by ChIP on chip (NimbleGen) and find a significant overlap, indicating that SIRT1 target genes are preferentially deregulated with age. Experiment Overall Design: Total neocortex RNA was hybridized on Affymetrix M430 2.0 arrays. RNA was isolated from five young (5 months) and 5 old (30 months) B6xC3 F1 mice, samples were hybridized individually.
transcription profiling by array, unknown experiment type
SIRT1 redistribution on chromatin promotes genomic stability but alters gene expression during aging. Oberdoerffer P, Michan S, McVay M, Mostoslavsky R, Vann J, Park SK, Hartlerode A, Stegmuller J, Hafner A, Loerch P, Wright SM, Mills KD, Bonni A, Yankner BA, Scully R, Prolla TA, Alt FW, Sinclair DA.