E-GEOD-1309 - Transcription profiling of human monoclonal cell lines with high or low levels of store-operated Ca2+ entry

Submitted on 14 April 2004, released on 3 August 2007, last updated on 27 March 2012
Homo sapiens
Samples (22)
Array (1)
Protocols (1)
This series of microarray experiments monitored the gene expression profiles for monoclonal cell lines (derived from HEK-293 parental cell culture) with high (H1, H15, H24, H36, H39) or low (L3, L28, L29) levels of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip. Two parallel hybridizations were done for each RNA preparation from each monoclonal cell line or from the parental HEK-293 cell culture.
Experiment types
transcription profiling by array, cell type comparison, co-expression
Gene expression profiles in HEK-293 cells with low or high store-operated calcium entry: can regulatory as well as regulated genes be identified?. Tatiana K Zagranichnaya, Xiaoyan Wu, Arpad M Danos, Mitchel L Villereal. Physiol Genomics 21(1):14-33 (2005)
Investigation descriptionE-GEOD-1309.idf.txt
Sample and data relationshipE-GEOD-1309.sdrf.txt
Processed data (1)E-GEOD-1309.processed.1.zip
Array designA-AFFY-33.adf.txt