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E-GEOD-13042 - Effect of P. gingivalis on human primary gingival epithelial cells

Status
Submitted on 2 October 2008, released on 16 May 2010, last updated on 4 May 2014
Organism
Homo sapiens
Samples (8)
Array (1)
Protocols (10)
Description
EXPERIMENT: Microarray expression profiles derived from the human primary gingival epithelial cells 24.0h after exposure to heat inactivated P. gingivalis ANIMAL MODEL: NON EXPOSURE: Human primary gingival epithelial cells (at 3rd passage) were exposed to heat inactivated P. gingivalis (MOI:100) at 90% confluence. Two types of gingival epithelial cells were used. One with Normal cytokine inducer type (at least 2 fold IL-6/TNF-alpha/IL-1ß when challenged with TLR2/4 agonists) and the other with diminished cytokine inducer type (no change in IL-6/TNF-alpha/IL-1ß when challenged with TLR2/4 agonists). INTERVAL: NON. PLATFORM: microRNA expression profile in gingival epithelial cells - miRCURY LNA™ microRNA Arrays (Exiqon). The RNA samples were subjected to microarray on 8/9/2007 Keywords = Human primary gingival epithelial cells Keywords = P. gingivalis Keywords = Periodontitis Keywords: Ordered The effect of heat inactivated P. gingivalison human primary gingival epithelial cells were assayed.
Experiment type
transcription profiling by array 
Contacts
Amar Singh <singhamarv@gmail.com>, Amar V Singh, Denis F Kinane, Manjunatha R Benakanakere
Citation
Modulation of TLR2 protein expression by miR-105 in human oral keratinocytes. Benakanakere MR, Li Q, Eskan MA, Singh AV, Zhao J, Galicia JC, Stathopoulou P, Knudsen TB, Kinane DF.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-13042.idf.txt
Sample and data relationshipE-GEOD-13042.sdrf.txt
Raw data (1)E-GEOD-13042.raw.1.zip
Processed data (1)E-GEOD-13042.processed.1.zip
Array designA-GEOD-7423.adf.txt
Links