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E-GEOD-12586 - Transcription profiling of human peripheral lymphocytes after exposure to cigarette smoke condensate in vitro

Status
Submitted on 27 August 2008, released on 15 September 2009, last updated on 1 May 2014
Organism
Homo sapiens
Samples (16)
Array (1)
Protocols (6)
Description
Little is known about alteration of the global gene expression by cigarette smoke (CS) and few biomarkers for smoking-related harm are available. We used Affymetrix HG-U133A GeneChips to measure the transcriptomes in eight cultured lymphocyte samples exposed to cigarette smoke condensate (CSC) in vitro . The in vitro exposure of lymphocytes to CSC significantly changed expression levels of 2,266 genes many of which biologically interacted. They included genes encoding for xenobiotic metabolism and oxidative stress-response (e.g. Nrf2 and AhR signaling pathways), inflammation/immune response (e.g. cytokines), apoptosis, cell cycle and tumorigenesis. However, the magnitude of expression responses for some genes showed high inter-individual variability. Experiment Overall Design: The goals of this study were to evaluate novel gene expression profiles and pathways affected by cigarette smoke condensate (CSC), and to identify potential biomarkers for cigarette smoke exposure and harm. To this end, we isolated the PBMC from eight light smokers, cultured the cells in vitro and exposed them to 2R4F CSC, then determined the gene expression profiles with Affymetrix microarray and analyzed alteration of global gene expression after exposure to CSC.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12586.idf.txt
Sample and data relationshipE-GEOD-12586.sdrf.txt
Raw data (1)E-GEOD-12586.raw.1.zip
Processed data (1)E-GEOD-12586.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-12586.eSet.r
Links