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E-GEOD-12585 - Transcription profiling of human healthy smokers lymphocytes

Status
Submitted on 27 August 2008, released on 15 September 2009, last updated on 1 May 2014
Organism
Homo sapiens
Samples (23)
Array (1)
Protocols (6)
Description
Little is known about alteration of the global gene expression by cigarette smoke (CS) and few biomarkers for smoking-related harm are available. We used Affymetrix HG-U133A GeneChips to measure the transcriptomes in lymphocytes of 23 current healthy smokers. When heavy smokers were compared with light smokers, 311 genes demonstrated a significant difference in mRNA expression of their blood lymphocytes. Experiment Overall Design: The goals of this study were to evaluate novel gene expression profiles and pathways affected by cigarette smoking, and to identify potential biomarkers for cigarette smoke exposure and harm. To this end, we isolated the PBMC from 10 heavy and 13 light smokers, determined their gene expression profiles with Affymetrix microarray and analyzed their relationship with cigarette smoking.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12585.idf.txt
Sample and data relationshipE-GEOD-12585.sdrf.txt
Raw data (1)E-GEOD-12585.raw.1.zip
Processed data (1)E-GEOD-12585.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-12585.eSet.r
Links