Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-12545 - Transcription profiling of mosue Gfi1+/+ and Gfi1-/- splenic B cells

Status
Submitted on 25 August 2008, released on 25 October 2008, last updated on 10 June 2011
Organism
Mus musculus
Samples (2)
Array (1)
Protocols (5)
Description
The zinc finger transcription factor growth-factor-independent-1 (Gfi1) has been involved in various cellular differentiation processes. Gfi1 acts as a transcriptional repressor and splicing control factor upon binding to cognate binding sites in regulatory elements of its target genes. Here, we report that Gfi1-deficient mice develop autoimmunity. Gfi1-deficient peripheral B-cells show a hyperproliferative phenotype, leading to expansion of plasma cells, increased levels of nuclear autoantibodies, and immunoglobulin deposition in brain and kidneys. Dysregulation of multiple transcription factors and cell-cycle control elements may contribute to B-cell dependent autoimmunity. Gfi1 thus emerges as a novel master-regulator restricting autoimmunity. Experiment Overall Design: Splenic B220+CD19+ CD138- B cells of 4 week old Gfi1+/+ and Gfi1-/- mice were isolated and RNA was extracted from one sample per group and microarray analysis was performed.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12545.idf.txt
Sample and data relationshipE-GEOD-12545.sdrf.txt
Raw data (1)E-GEOD-12545.raw.1.zip
Processed data (1)E-GEOD-12545.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-12545.eSet.r
Links