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E-GEOD-12506 - Transcription profiling of mouse Foxp3- CD25- regulatory T cells

Status
Released on 25 October 2008, last updated on 1 May 2014
Organism
Mus musculus
Samples (21)
Array (1)
Protocols (5)
Description
The concept of immune regulation/suppression has been well-established. With thymus-derived CD4 CD25 regulatory T (TR) cells, it became clear that a variety of additional peripherally induced TR cells play vital roles in protection from many harmful immune responses including intestinal inflammation. In the present study, we have analyzed in vivo-induced Ag-specific CD4 TR cells with respect to their molecular and functional phenotype. By comparative genomics we could show that these Ag-specific TR cells induced by chronic Ag stimulation in vivo clearly differ in their genetic program from naturally occurring thymus-derived CD4 CD25 TR cells. This distinct population of induced TR cells express neither CD25 nor the TR-associated transcription factor Foxp3. Strikingly, CD25 is not even up-regulated upon stimulation. Despite the lack in Foxp3 expression, these in vivo-induced CD25 TR cells are able to interfere with an Ag-specific CD8 T cell-mediated intestinal inflammation without significant increase in CD25 and Foxp3 expression. Thus, our results demonstrate that in vivo-induced Ag-specific TR cells represent a distinct population of Foxp3 CD25 TR cells with regulatory capacity both in vitro and in vivo. Experiment Overall Design: To define the molecular signature of Ag-specific in vivo-induced dtg CD25 TR cells in comparison to naturally occurring CD25 TR cells, we performed comparative gene expression profiling by Affymetrix microarray analysis. Sorted splenic wild-type (WT) TR cells, stg TR cells, dtg CD25- TR cells, dtg CD25+ TR cells, in vitro-stimulated stg 16h TA cells, stg 3d TA cells as well as stg TN cells, were included in the experiment and analyses were performed in triplicates.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
Chronic antigen stimulation in vivo induces a distinct population of antigen-specific Foxp3 CD25 regulatory T cells. Wiebke Hansen, Astrid M Westendorf, Simone Reinwald, Dunja Bruder, Stefanie Deppenmeier, Lothar Groebe, Michael Probst-Kepper, Achim D Gruber, Robert Geffers, Jan Buer. J Immunol 179(12):8059-68 (2007)
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-12506.idf.txt
Sample and data relationshipE-GEOD-12506.sdrf.txt
Raw data (1)E-GEOD-12506.raw.1.zip
Processed data (1)E-GEOD-12506.processed.1.zip
Array designA-AFFY-36.adf.txt
R ExpressionSetE-GEOD-12506.eSet.r
Links