E-GEOD-12207 - Transcription profiling of Pseudomonas aeruginosa reveals biofilms and type III secretion are not mutually exclusive
Submitted on 22 July 2008, released on 13 November 2008, last updated on 10 June 2011
Biofilm formation and type III secretion have been shown to be reciprocally regulated in P. aeruginosa, and it has been suggested that factors related to acute infection may be incompatible; with biofilm formation. We investigated how growth conditions influence the production of virulence factors by studying the inter-relationships between colonies, biofilms and planktonic cells. We found that biofilms in our growth conditions express the type III secretion and these lifestyles are therefore not mutually exclusive in P. aeruginosa. Experiment Overall Design: Pseudomonas aeruginosa cells grown in five different conditions were analysed with three biological replicates for each sample. The five different conditions were planktonic cells in exponential phase, planktonic cells in stationary phase, colonies on agar plates incubated for 15 or 40 hours and biofilms in a continuous flow system after three days of growth.
transcription profiling by array, unknown experiment type