E-GEOD-11954 - Transcription profiling by array of human growing and senescent activated hepatic stellate cells

Status
Released on 29 August 2008, last updated on 9 October 2012
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (3)
Description
Cellular senescence is an irreversible proliferative arrest and can be triggered in many cell types in response to diverse forms of cellular damage or stress. We used microarrays to compare gene expression profile between growing and senescent human activated hepatic stellate cells. Experiment Overall Design: Two separate preparation of activated hepatic stellate cells were treated with DNA damaging agent to induce senescence or vechicle to remain growing. Experiment Overall Design: RNA was extracted from both replications and used for hybridization on Affymetrix arrays to determine expression differences.
Experiment type
transcription profiling by array 
Contact
Citation
Senescence of activated stellate cells limits liver fibrosis. Krizhanovsky V, Yon M, Dickins RA, Hearn S, Simon J, Miething C, Yee H, Zender L, Lowe SW.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11954.idf.txt
Sample and data relationshipE-GEOD-11954.sdrf.txt
Raw data (1)E-GEOD-11954.raw.1.zip
Processed data (1)E-GEOD-11954.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-11954.eSet.r
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