E-GEOD-11954 - Transcription profiling by array of human growing and senescent activated hepatic stellate cells
Released on 29 August 2008, last updated on 9 October 2012
Cellular senescence is an irreversible proliferative arrest and can be triggered in many cell types in response to diverse forms of cellular damage or stress. We used microarrays to compare gene expression profile between growing and senescent human activated hepatic stellate cells. Experiment Overall Design: Two separate preparation of activated hepatic stellate cells were treated with DNA damaging agent to induce senescence or vechicle to remain growing. Experiment Overall Design: RNA was extracted from both replications and used for hybridization on Affymetrix arrays to determine expression differences.
transcription profiling by array
Senescence of activated stellate cells limits liver fibrosis. Krizhanovsky V, Yon M, Dickins RA, Hearn S, Simon J, Miething C, Yee H, Zender L, Lowe SW.