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E-GEOD-11898 - Transcription profiling of mouse primary mesangial cells stimulated with DNA and RNA ligands

Status
Submitted on 26 June 2008, released on 21 October 2008, last updated on 10 June 2011
Organism
Mus musculus
Samples (9)
Array (1)
Protocols (6)
Description
Extrarenal viral infections commonly trigger glomerulonephritis mostly in association with immune complex disease. The immunoglobulin component of immune complexes can activate glomerular cell Fc receptors but whether complexed viral nucleic acids contribute to glomerular inflammation remains unknown. Glomerular mesangial cells express TLR3 but lack TLR7-9, hence, it is unclear whether mesangial cells can recognize and respond to viral ssRNA or DNA. Here we studied the immune responses activated by 3P-RNA (5'Triphosphate RNA) and Non-CpG DNA (Double stranded DNA) in primary mesangial cells (PMC). We used microarrays to detail the global programme of gene expression that induced by 3P-RNA and Non-CpG DNA. Experiment Overall Design: PMC were stimulated with 3P-RNA and Non-CpG DNA for 6 hours and then total RNA was isolated for hybridization to MOE430_2 arrays.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11898.idf.txt
Sample and data relationshipE-GEOD-11898.sdrf.txt
Raw data (1)E-GEOD-11898.raw.1.zip
Processed data (1)E-GEOD-11898.processed.1.zip
Experiment designE-GEOD-11898.biosamples.png, E-GEOD-11898.biosamples.svg
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-11898.eSet.r
Links