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E-GEOD-11884 - Transcription profiling of mouse naive T cells isolated from CD4+ CD62L+ CD44- T cells were isolated from Fur flox/flox and CD4 cre Fur flox/flox mice reveals T cell-expressed proprotein convertase furin is essential for maintenance of peripheral tolerance

Status
Released on 21 October 2008, last updated on 10 June 2011
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (5)
Description
Furin is a proprotein convertase induced in activated T cells, reported to processes the anti-inflammatory cytokine TGFb-1. Herein, we show that conditional deletion of furin in T cells allowed for normal T cell development but impaired the function of regulatory T cells and effector cells, which produced less TGFb-1. Furin-deficient Treg cells, were less protective in a T cell transfer colitis model and failed to induce Foxp3 in normal T cells. Furin-deficient effector cells were inherently overly active and were resistant to suppressive activity of wild-type Tregs. Thus, our results indicate that furin is indispensable in maintaining peripheral tolerance, which is due, at least in part, to its nonredundant, essential function in regulating TGFb-1 production. Targeting furin has emerged as a strategy in malignant and infectious disease. The current work suggests that inhibiting furin might activate immune responses, but may result in a breakdown in peripheral tolerance. Experiment Overall Design: Naive CD4+ CD62L+ CD44- T cells were isolated from Fur flox/flox and CD4 cre Fur flox/flox mice. Replicated samples were achieved for wild type and knockout conditions.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11884.idf.txt
Sample and data relationshipE-GEOD-11884.sdrf.txt
Raw data (1)E-GEOD-11884.raw.1.zip
Processed data (1)E-GEOD-11884.processed.1.zip
Experiment designE-GEOD-11884.biosamples.png, E-GEOD-11884.biosamples.svg
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-11884.eSet.r
Links