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E-GEOD-11867 - Expression Profiling of Dll1 mutant mouse lines on different genetic background

Status
Released on 1 January 2009, last updated on 1 May 2014
Organism
Mus musculus
Samples (172)
Array (1)
Protocols (8)
Description
The evolutionarily conserved Delta-Notch cell-cell signal-transduction pathway regulates the determination of various cell fates, which are important for the morphogenesis and development of numerous organs and tissues in many vertebrates and invertebrates. The Dll1 gene was mutated by homologous recombination in embryonic stem cells such that amino acids 2-116 were replaced with an in-frame fusion of the lacZ gene of E.coli. Mouse Dll1 genomic clones were isolated from a strain 129/SvPas library. The replacement vector was constructed by inserting a 4 kb ClaI/XhoI fragment, which encodes a part of the beta-galactosidase gene followed by the PGK-neo cassette, and a 2.6 kb XhoI/EcoRI fragment as the 3’ homologous region of the replacement vector into pKS+. This vector was linearized with SalI and ClaI, and a 4.1 kb SalI/NcoI as the 5’ homologous region plus an NcoI/ClaI fragment (800 bp) completing the b-galactosidase gene, were inserted, resulting in the complete replacement vector. This vector was linearized with SalI and electroporated in R1 embryonic stem cells. Correctly targeted clones were identified and verified by Southern blot analysis using external probes from the 3’ and 5’ region of the targeted area, and were injected into C57BL/6J embryo to obtain germline transmission. The two analysed Dll1 mutant mouse lines carry the same mutation but are on different genetic background: 1. Heterozygous F1-animals were backcrossed several times to the 129SV/J wild type; 2. Heterozygous F1-animals were outcrossed 11 generation to C3HeB/FeJ wild type Four organs (liver, spleen, thymus, brain) of two Dll1 mutant mouse lines on different genetic background carrying the same mutation were analysed by cDNA microarray technology. Experiment include 4-5 biological replicates for reference (wildtype) and mutant animals. Up to 4 technical replicates for each mutant mouse were performed. As reference pooled RNA was used. 50% of the chip hybridisations are dye sway experiments.
Experiment type
transcription profiling by array 
Contacts
Marion Horsch <geo@ncbi.nlm.nih.gov>, Gerhard Przemeck, Isabel Rubia-Aliaga, Johannes Beckers
Citation
Dll1 haploinsufficiency in adult mice leads to a complex phenotype affecting metabolic and immunological processes. Rubio-Aliaga I, Przemeck GK, Fuchs H, Gailus-Durner V, Adler T, Hans W, Horsch M, Rathkolb B, Rozman J, Schrewe A, Wagner S, Hoelter SM, Becker L, Klopstock T, Wurst W, Wolf E, Klingenspor M, Ivandic BT, Busch DH, Beckers J, Hrabé de Angelis M. , PMID:19562077
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11867.idf.txt
Sample and data relationshipE-GEOD-11867.sdrf.txt
Raw data (1)E-GEOD-11867.raw.1.zip
Processed data (1)E-GEOD-11867.processed.1.zip
Array designA-GEOD-4937.adf.txt
Links