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E-GEOD-11735 - Mutant analysis of C. elegans small RNAs with 5p monophosphates

Status
Released on 15 June 2008, last updated on 2 June 2014
Organism
Caenorhabditis elegans
Samples (3)
Protocols (4)
Description
These datasets profile the expression of small RNAs with 5p monophosphates and 3p hydroxyls (including miRNAs, 21U-RNAs) in young adult worms from the wt, prg-1 mutant, and fog-2 mutant backgrounds. These datasets were prepared in the Craig C. Mello laboratory using T4 RNA ligase 1. Keywords: miRNA and 21U-RNA discovery and mutant expression profiling CE-mutProfile-5pDependent-Illumina 1 flowcell each for the wt, prg-1 mutant, and fog-2 mutant
Experiment type
RNA-seq of non coding RNA 
Contacts
James Graham Ruby <geo@ncbi.nlm.nih.gov>, Craig C Mello, Daniel A Chaves, Darryl Conte, David P Bartel, Gary P Schroth, J G Ruby, James C Carrington, Jessica J Vasale, Julie M Claycomb, Kristin D Kasschau, Noah Fahlgren, Pedro J Batista, Rosaria Chiang, Shenghua Duan, Shujun Luo, Weifung Gu
Citation
PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. Batista PJ, Ruby JG, Claycomb JM, Chiang R, Fahlgren N, Kasschau KD, Chaves DA, Gu W, Vasale JJ, Duan S, Conte D Jr, Luo S, Schroth GP, Carrington JC, Bartel DP, Mello CC. , PMID:18571452
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-11735.idf.txt
Sample and data relationshipE-GEOD-11735.sdrf.txt
Processed data (1)E-GEOD-11735.processed.1.zip
Links