E-GEOD-11625 - Ecdysone Response Timecourse in Kc Cells
Submitted on 29 May 2008, released on 12 June 2008, last updated on 1 May 2014
Kc167 Cells were plated at 0.5x106/ml and grown at 23.50C. They were treated with 20-HE (Sigma) at a final concentration of 5x10-7M. The reference sample was total RNA isolated at 0 hr. after treatment. The experimental samples were total RNA isolated from cells treated for 1, 3, 6, 12, 24, and 48 hours. Samples were taken at 0hr, 1hr, 3hr, 6hr, 12hr, 24hr and 48hr. Briefly, cells were spun down and Total mRNA was extracted with OligodT (Ambion) nd reverse transcribed in the presence of oligodeothymidine and random hexamers and labeled to the whole genome in-house cDNA microarray and ecdysone responsive genes were identified. Ecdysone responsive genes were identified at each timepoint using a Limma t-test.
transcription profiling by array
Christopher Mason, Kevin P White, Ling V Sun, Sujun Hua, Tong-Ruei Li, Zareen Gauhar
Genomic mapping of binding regions for the Ecdysone receptor protein complex. Gauhar Z, Sun LV, Hua S, Mason CE, Fuchs F, Li TR, Boutros M, White KP.