E-GEOD-11597 - Transcription profiling of chicken B cell lymphoma (DT40) cell lines
Submitted on 29 May 2008, released on 13 November 2008, last updated on 1 May 2014
Global gene expression profiling of the avian B-lymphoma DT40 cell line was used as a model to differentiate among Btk KO and Btk KO cells reconstituted with human Btk. Differences in the gene expression pattern showed statistically significant changes between parental DT40 and all the Btk KO cell populations irrespective of whether they are reconstituted or not. These results imply that in the process of generating a knockout cell line, subclones are selected, which have multiple changes in their gene expression pattern (p<0.01). Experiment Overall Design: DT40 cells along with the mutants were grown at various time points in different batches of fetal calf serum with or without any stimulation. All experiments were repeated ten times and then polled together as one sample. In total 28 samples were used RNA extraction and hybridization on Affymetrix microarrays.
transcription profiling by array, unknown experiment type
Expression profiling of chicken DT40 lymphoma cells indicates clonal selection of knockout and gene reconstituted cells. Blomberg Nawaz, Kurosaki Lindvall, Smith.