E-GEOD-11458 - Lactate modulates gene expression in human mesenchymal stem cells
Released on 15 May 2010, last updated on 2 May 2014
Wound healing is associated with high rates of cell replication and lactate accumulation even under normoxic and hyperoxic conditions. Lactate accounts for various effects in tissue regeneration, such as collagen synthesis, angiogenesis, modulation of cytokine patterns and as recently shown for stem cell homing. Its influence on genes involved in cell replication has not been shown yet. Therefore, the effect of lactate considering genes involved in different cellular processes was investigated. Human umbilical vein endothelial cells (HUVEC) were cultured and incubated with lactate for different periods of time. Gene expression analysis was performed using custom-designed oligonucleotide microarrays. Keywords: Human gene expression study Reference design with Cy3 labeled uniRNA and Cy5 labeled sample RNA.
transcription profiling by array
Derek Zieker, Katharina Buck, Kay Nieselt
Lactate modulates gene expression in human mesenchymal stem cells. Zieker D, Schäfer R, Glatzle J, Nieselt K, Coerper S, Kluba T, Northoff H, Königsrainer A, Hunt TK, Beckert S. , Europe PMC 18273635