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E-GEOD-11187 - Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum

Status
Released on 17 April 2008, last updated on 27 March 2012
Organism
Bradyrhizobium japonicum
Samples (10)
Array (1)
Protocols (7)
Description
Analysis of a Bradyrhizobium japonicum pmtA mutant. PmtA catalyzes the first of three consecutive methylation reactions leading to phosphatidylcholine (PC) formation in B. japonicum. Disruption of the pmtA gene results in a significantly reduced PC content causing a defect in symbiosis with the soybean host. This study provides the first insight into global transcriptomic changes of a bacterial phosphatidylcholine biosynthesis mutant. Cells of the pmtA mutant and the wild type were grown to mid-exponential phase in full medium (PSY) under aerobic culture conditions. Keywords: genetic modification Comparative analyis of the B. japonicum pmtA mutant and the wild type grown under aerobic culture conditions.
Experiment type
unknown experiment type 
Contacts
Stefan Zoller <szoller@fgcz.ethz.ch>, A Lindemann, F Narberhaus, G Pessi, H Hennecke, H.M Fischer, S Hacker, S Mesa
Citation
Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum. Hacker S, Gödeke J, Lindemann A, Mesa S, Pessi G, Narberhaus F. , PMID:18446372
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11187.idf.txt
Sample and data relationshipE-GEOD-11187.sdrf.txt
Raw data (1)E-GEOD-11187.raw.1.zip
Processed data (1)E-GEOD-11187.processed.1.zip
Array designA-GEOD-3401.adf.txt
R ExpressionSetE-GEOD-11187.eSet.r
Links