6 protocols
AccessionType
feature_extraction
Title: Affymetrix CEL analysis. Description:
hybridization
(Parameters: Extracted product = total_RNA, Amplification = none)
 
grow
mouse embryonic lungs were isolated at E18.5 of development
labeling
Standard Affymetrix protocol using 5 micorgrams total RNA, Superscript II RT. Second strand synthesis and in vitro amplification and incorporation with biotynalted CTP and UTP.
nucleic_acid_extraction
Trizol