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E-GEOD-11129 - ler vs pop2-1: gaba over-accumulation effects-Transcriptomic analysis of pop2-1 mutant in Arabidopsis thaliana

Status
Submitted on 9 April 2008, released on 11 April 2009, last updated on 1 May 2014
Organism
Arabidopsis thaliana, Populus x canadensis
Samples (24)
Array (1)
Protocols (34)
Description
au05-03_gaba - ler vs pop2-1: gaba over-accumulation effects - The analysis aims at identifying genes that are differentially regulated by the over-accumulation of GABA observed in the mutant pop2-1 in response to treatment with exogenous GABA and that may explain the singular phenotype of the mutant in this condition. Designed experiment consisted in comparison of transcriptomes of Arabidopsis thaliana Landsberg erecta ecotype and its mutant pop2-1 (impaired in GABA transaminase activity) during a kinetic of endogenous GABA accumulation. For this purpose, 10-day-old plants grown on half strength Hoagland's agar medium were transferred to agar plates supplemented with 1 mM GABA. We isolated RNA from plants treated for 0, 1 and 4 days. Treatments were made in duplicate. Keywords: gene knock-out, time course 6 dye-swap - CATMA arrays
Experiment type
transcription profiling by array 
Contacts
Agnes Yu <yu@evry.inra.fr>, Carole DELEU, Jean-Pierre P Renou, Marie-Laure L Martin-Magniette
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-11129.idf.txt
Sample and data relationshipE-GEOD-11129.sdrf.txt
Processed data (1)E-GEOD-11129.processed.1.zip
Experiment designE-GEOD-11129.biosamples.png, E-GEOD-11129.biosamples.svg
Array designA-GEOD-4346.adf.txt
Links