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E-GEOD-10137 - Transcription profiling of human chronic lymphocytic leukemia samples to improve prognosis and predict therapeutic response

Status
Submitted on 10 January 2008, released on 11 November 2009, last updated on 1 May 2014
Organism
Homo sapiens
Samples (40)
Array (1)
Protocols (5)
Description
Chronic lymphocytic leukemia (CLL) is a heterogeneous malignancy, characterized by a variable clinical course. While clinical and laboratory parameters are increasingly being used to refine prognosis, they do not accurately predict response to commonly used therapy. We used gene expression profiling to generate and further refine prognostic and predictive markers. Genomic signatures that reflect progressive disease and responses to chemotherapy or chemo-immunotherapy were created using cancer cell lines and patient leukemia samples. We validated these signatures using independent clinical data from four separate cohorts representing a total of 301 CLL patients. A prognostic genomic signature created from patient leukemic cell gene expression data coupled with clinical parameters could statistically differentiate patients with stable or progressive disease in the training dataset. The progression signature was then validated in two independent datasets, demonstrating a capacity to accurately identify patients at risk for progressive disease. In addition, two distinct genomic signatures that predict response to chlorambucil or pentostatin, cyclophosphamide, and rituximab were also generated and were shown to accurately distinguish responding and non-responding CLL patients. Microarray analysis of CLL patients’ lymphocytes can be used to refine prognosis and predict response to different therapies. These results have direct implications for standard and investigational therapeutics in CLL patients. Experiment Overall Design: For the predictive genomic signature or response to pentostatin, cyclophosphamide, and rituximab, 20 CLL leukemia samples were used in the training set, and 20 CLL leukemia samples were used in the validation set
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
A Genomic Approach to Improve Prognosis and Predict Therapeutic Response in Chronic Lymphocytic Leukemia. Weinberg Friedman, Goodman Barry, Bond Volkheimer, Jiang Chen, Gockerman Moore, Decastro Diehl, Nevins Potti.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-10137.idf.txt
Sample and data relationshipE-GEOD-10137.sdrf.txt
Raw data (1)E-GEOD-10137.raw.1.zip
Processed data (1)E-GEOD-10137.processed.1.zip
Experiment designE-GEOD-10137.biosamples.png, E-GEOD-10137.biosamples.svg
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-10137.eSet.r
Links