E-GEOD-10059 - Microarray assay of the genetic response of Picea abies to Heterobasidion annosum infection - Loop2
Submitted on 2 January 2008, released on 3 May 2008, last updated on 1 May 2014
The hypothesis of the experiment is that infected trees of high resistance express a wider variety of resistance genes than infected trees of low resistance, and that the level of expression of these resistance genes differs between infected and healthy branches. Also, some genes highly expressed in the infected state not expressed in the healthy state may be in response to the wounding rather than the actual infection. By comparing these expressions to that of wounded, uninfected branches, this could also be clarified. Three different Picea abies clones, of increasing resistance to fungal attack, have been infected. For all clones, three rametes have been infected with Heterobasidion of 1 month, by means of cutting the cambium and inoculating a piece of fungus growing in agar in the wound. For the low and medium resistant clone, a fourth ramete has been cut and inoculated with agar not containing any fungal material.
unknown experiment type
Chris Smith, Ake Olson, Aleksandra Adomas, Bo Karlsson, Chris P Smith, Debbie Craig, Frederick O Asiegbu, Gunnilla Swedjemark, Guosheng Li, Jan Stenlid, Len van Zyl, Mårten Lind, Roger Finlay, Ron Sederoff