E-FPMI-9 - Transcription profiling of peripheral blood monocytes from healthy humans and from those with Chronic Granulamatous Disease in response to stimulation with peptidoglycan and lipopolysaccharide
Released on 5 November 2008, last updated on 1 May 2014
Persons with Chronic Granulomatous Disease, are susceptible to a narrow range of infections resulting from the failure of cells to generate toxic reactive oxygen species (ROS) required for phagocytice oxidative killing of microorganisms. For unknown reasons, many inflammatory conditions (inflammatory bowel disease, periodontal inflammation, granulomatous obstruction of the urinary and gastrointestinal tract and “sterile” inflammation of the lungs and other organs) are also associated with the disease. The goal of this study is to investigate the role of ROS in the regulation of inflammatory and immunity genes induced by Toll-like Receptors (TLRs). Transcriptional responses to peptidoglycan or lipopolysaccharide (TLR2/4 agonists) were evaluated at 4 and 24 hrs in peripheral blood monocytes (PBM) from three males with CGD compared to PBM from 5 healthy individuals.
transcription profiling by array, compound treatment, disease state, time series
ROS-deficient monocytes have aberrant gene expression that correlates with inflammatory disorders of chronic granulomatous disease. Brown KL, Bylund J, MacDonald KL, Song-Zhao GX, Elliott MR, Falsafi R, Hancock RE, Speert DP. Clin Immunol 129(1):90-102 (2008)