E-FPMI-6 - Transcription profiling of human monocytic cells THP-1 stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours
Released on 7 December 2006, last updated on 1 May 2014
The objective of the study was to evaluate transcriptional response of endotoxin-stimulated human monocytic cells in presence or absence of host defense peptide LL-37 at low physiologically relevant concentrations. Human monocytic cells THP-1 were stimulated with LPS (10ng/ml) in presence or absence of LL-37 (5 ug/ml), as well as with the peptide alone, for 4 hours. The trends of LPS-induced altered gene expression in presence of the peptide were further validated by quantitative real-time PCR.
transcription profiling by array, compound treatment
Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide. Mookherjee, Neeloffer; Wilson, Heather L.; Doria, Silvana; Popowych, Yurij; Falsafi, Reza; Yu, Jie (Jessie); Li, YueXin; Veatch, Sarah; Roche, Fiona M.; Brown, Kelly L.; Brinkman, Fiona S. L.; Hokamp, Karsten; Potter, Andy; Babiuk, Lorne A.; Griebel, Philip J.; Hancock, Robert E. W. J Leukoc Biol 80(6):1563 (2006), PMID:16943385