4 protocols
AccessionType
nucleic acid sequencing protocol
ILLUMINA sequencing using Illumina Genome Analyzer II. http://www.illumina.com/support/sequencing/sequencing_instruments.ilmn
nucleic acid library construction protocol
Genomic DNA was extracted, sonicated and isolated by CxxC affinity purification (CAP) using recombinant CXXC from mMBD1 as described in Illingworth et al., PLoS Biology, 2008.
ChIP
Chromatin Immunoprecipitation with H3K4me3, H3Ac, ES-E14TG2a, RNA Polymerase II antibodies and input DNA for controls. http://www.protocol-online.org/cgi-bin/prot/jump.cgi?ID=4106
nucleic acid library construction protocol
Genomic DNA was extracted, sonicated and isolated with MBD affinity purification (MAP) using recombinant MBD from hMeCP2 as described in Skene et al., Moll Cell 2010.