E-CBIL-9 - Transcription profiling of rat pancreatic beta-cells in response to cytokine exposure and NF-kappa B activation blocking

Status
Released on 16 June 2006, last updated on 12 October 2011
Organism
Rattus norvegicus
Samples (24)
Array (1)
Protocols (9)
Description
Type 1 diabetes mellitus results from an autoimmune destruction of pancreatic beta-cells. Based on findings suggesting NF-kappa B plays a role in beta cell apoptosis, we blocked NF-kappa B activation in cytokine-exposed FACS sorted beta cells by a recombinant adenovirus (AdI kappa B((SA)2)) containing an inhibitor of NF kappa B alpha (I kappa Bac) super-repressor (S32A/S36A). The expression profile was then analyzed with the Affymetrix RG U34a microarray.
Experiment types
transcription profiling by array, cellular modification, compound treatment
Contact
Citation
A comprehensive analysis of cytokine-induced and nuclear factor-kappa B-dependent genes in primary rat pancreatic beta-cells. Cardozo AK, Heimberg H, Heremans Y, Leeman R, Kutlu B, Kruhoffer M, Orntoft T, Eizirik DL.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-CBIL-9.idf.txt
Sample and data relationshipE-CBIL-9.sdrf.txt
Processed data (1)E-CBIL-9.processed.1.zip
Experiment designE-CBIL-9.biosamples.png, E-CBIL-9.biosamples.svg
Array designA-AFFY-18.adf.txt
Links