E-CBIL-8 - Transcription profiling of rat pancreatic beta-cells in response to INF gamma and/or IL-1 beta treatment for 6 or 24 hours

Status
Released on 27 May 2006, last updated on 12 October 2011
Organism
Rattus norvegicus
Samples (30)
Array (1)
Protocols (9)
Description
Type 1 diabetes is an autoimmune disease resulting from the selective destruction of insulin-producing beta-cells. Beta cell culture for 6-9 days in the presence of IL-1 beta and interferon (INF) gamma leads to apoptosis. Primary rat beta-cells were FACS purified and exposed for 6 or 24 h to control condition, IL-1 beta + INF gamma, or IL-1 beta alone (24 h only). The gene expression profile was analyzed by hybridization in duplicate to the Affymetrix RG U34A microarray.
Experiment types
transcription profiling by array, compound treatment, time series
Contacts
Citation
Identification of novel cytokine-induced genes in pancreatic beta-cells by high-density oligonucleotide arrays. Alessandra K. Cardozo, Mogens Kruhøffer, Ruth Leeman, Torben Ørntoft, and Décio L. Eizirik.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-CBIL-8.idf.txt
Sample and data relationshipE-CBIL-8.sdrf.txt
Processed data (1)E-CBIL-8.processed.1.zip
Experiment designE-CBIL-8.biosamples.png, E-CBIL-8.biosamples.svg
Array designA-AFFY-18.adf.txt
Links