E-CBIL-8 - Transcription profiling of rat pancreatic beta-cells in response to INF gamma and/or IL-1 beta treatment for 6 or 24 hours
Released on 27 May 2006, last updated on 12 October 2011
Type 1 diabetes is an autoimmune disease resulting from the selective destruction of insulin-producing beta-cells. Beta cell culture for 6-9 days in the presence of IL-1 beta and interferon (INF) gamma leads to apoptosis. Primary rat beta-cells were FACS purified and exposed for 6 or 24 h to control condition, IL-1 beta + INF gamma, or IL-1 beta alone (24 h only). The gene expression profile was analyzed by hybridization in duplicate to the Affymetrix RG U34A microarray.
transcription profiling by array, compound treatment, time series
Identification of novel cytokine-induced genes in pancreatic beta-cells by high-density oligonucleotide arrays. Alessandra K. Cardozo, Mogens Kruhøffer, Ruth Leeman, Torben Ørntoft, and Décio L. Eizirik.