E-CBIL-6 - Transcription profiling of HNF4 alpha null mice to investigate the phenotype of dis-regulated insulin secretion and abnormal beta cell growth
Released on 5 September 2006, last updated on 2 June 2014
The aim of this experiment was to use microarray analysis to examine the phenotype of dis-regulated insulin secretion and abnormal beta cell growth in HNF4 alpha null mice. These mice show impaired glucose tolerance and elevated fasting and fed plasma insulin levels. Rana Gupta from Klaus Kaestner's Lab extracted RNA from isolated islets. Three controls and five mutants were provided for the study.
transcription profiling by array, dye swap, genetic modification
Control of Beta-cell Gene Expression by HNF-4alpha. Rana K. Gupta, Regina K. Gorski, Peter White, Olga T. Hardy, John E. Brestelli, Guang Chen, Christian J. Stoeckert, Jr. and Klaus H. Kaestner.