E-CBIL-5 - Transcription profiling of mouse islet growth after partial pancreatectomy and exendin-4 Treatment
Released on 1 December 2005, last updated on 12 October 2011
Diabetes mellitus results from an inadequately functioning beta-cell mass. In the adult pancreas, beta-cell mass is dynamic, increasing to meet metabolic demands and decreasing with metabolic or injury insults. Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor agonist that augments beta-cell mass by increasing beta-cell neogenesis and proliferation and by reducing apoptosis. We utilized a cDNA microarray approach to identify genes that are differentially regulated during islet growth after Ex-4 treatment or a partial pancreatectomy (Ppx). Mice underwent 50% Ppx or sham operation and received Ex-4 or vehicle every 24 hours. cDNA prepared from total pancreatic RNA isolated at 12, 24 and 48 hrs after surgery was hybridized to the PancChip 4.0 microarray.
transcription profiling by array, compound treatment, stimulus or stress, time series
Identification of transcriptional targets during pancreatic growth after partial pancreatectomy and exendin-4 treatment. De Leon, Diva D.; Farzad, Cyrus; Crutchlow, Michael F.; Brestelli, John; Tobias, John; Kaestner, Klaus H.; Stoffers, Doris A.