E-CBIL-35 - Transcription profiling of rat insulinoma (INS-1 derived) cell lines that vary in their glucose responsiveness
Submitted on 24 July 2007, released on 11 September 2007, last updated on 1 May 2014
The aim of this study was to identify the gene expression changes associated with glucose responsiveness in Rat INS-1 derived cell lines. RNA was prepared from cell lines which were shown to be highly glucose responsive and also lines which were shown to be poorly glucose responsive. Dr. Chris Newgard's lab quantified the RNA and sent it frozen in water. 3 biological replicates per condition were sent for the following conditions: (i) 832/13 and 833/15, robust glucose responsiveness; (ii)832/1 and 832/2 showed poor glucose responsiveness.
transcription profiling by array, cell type comparison
Functional genomics of the beta-cell: SCHAD regulates insulin secretion independent of K+ currents. Hardy O.T., Hohmeier H.E., Becker T.C., Manduchi E., Doliba N.M., Gupta R.K., White P., Stoeckert C.J. Jr., Matschinsky F.M., Newgard C.B., Kaestner K.H. Mol Endocrinol (2006)