E-CBIL-29 - Transcription profiling of inducible Ngn3 mouse embryonic stem cells
Released on 29 June 2007, last updated on 12 October 2011
16 embryonic stem cell (ESC) samples (8 groups in duplicate) were processed for microarray analysis using the Affymetrix Mouse Genome 430 2.0 GeneChip. The samples included the parental Ainv15 ESC line (1) undifferentiated (Ainv15 ESC), (2) differentiated for 3 days as embryoid bodies (Ainv15 EB3) or (3) for 10 days as embryoid bodies (Ainv15 EB10). The remaining samples included tetracycline inducible Ngn3 ESC line derived from the parental Ainv15 ESC line (4) after differentiation and addition of doxycycline for 3 days without embryoid body formation (Ngn3 ES3 ON), (5 and 6) after differentiation as embryoid bodies for 3 days with (Ngn3 EB3 ON) or without (Ngn3 EB3 OFF) doxycycline, and (7 and 8) after differentiation as embryoid bodies for 10 days with (Ngn3 EB10 ON) or without (Ngn3 EB10 OFF) doxycycline.
transcription profiling by array, compound treatment, genetic modification, growth condition
Differentiation of embryonic stem cells conditionally expressing neurogenin 3. Treff Nathan R, Vincent Robert K, Budde Melisa L, Browning Victoria L, Magliocca Joseph F, Kapur Vivek, Odorico Jon S. Stem Cells 24(11):2529-37 (2006)